Hotspot

psaE

LSC · 19693-19890 · 198 bp

Back to Stephanodiscus

Pi 0.00673

MarkerSeek score 30.70

Primer available yes

Label psaE

Feature Metadata

label_name: psaE
feature_type: gene
region: LSC
length_bp: 198
variable_sites: 2
pi: 0.00673
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 30.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaE_p1	CAAGCAGAAGGTCACAGGTT	TCCAATTTGCAGGACAAGCT	50.0	45.0	58.0	58.0	1173-1180 bp	1.0	81.6
psaE_p2	GCAGAAGGTCACAGGTTCAA	TCCAATTTGCAGGACAAGCT	50.0	45.0	58.0	58.0	1170-1177 bp	1.0	81.6
psaE_p3	AACTCAGCGGTAGAGTGTCT	TCCAATTTGCAGGACAAGCT	50.0	45.0	58.1	58.0	1199-1206 bp	1.0	81.1
psaE_p4	TACGCCCGTTAAATGCTGAA	TCCAATTTGCAGGACAAGCT	45.0	45.0	57.9	58.0	1552-1559 bp	1.0	80.9
psaE_p5	TTACGCCCGTTAAATGCTGA	TCCAATTTGCAGGACAAGCT	45.0	45.0	57.9	58.0	1553-1560 bp	1.0	80.9