Hotspot

dnaK

SSC · 88326-90122 · 1797 bp

Back to Stephanodiscus

Pi 0.00853

MarkerSeek score 50.00

Primer available yes

Label dnaK

Feature Metadata

label_name: dnaK
feature_type: gene
region: SSC
length_bp: 1797
variable_sites: 23
pi: 0.00853
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
dnaK_p1	AAGACCGGATTGCCAAACTT	CAGCAGGATCATTCACACGA	45.0	50.0	58.0	58.0	2987 bp	1.0	81.4
dnaK_p2	AAGACCGGATTGCCAAACTT	TCAGCAGGATCATTCACACG	45.0	50.0	58.0	58.0	2988 bp	1.0	81.4
dnaK_p3	AGACCGGATTGCCAAACTTT	CAGCAGGATCATTCACACGA	45.0	50.0	58.0	58.0	2986 bp	1.0	81.4
dnaK_p4	AGACCGGATTGCCAAACTTT	TCAGCAGGATCATTCACACG	45.0	50.0	58.0	58.0	2987 bp	1.0	81.4
dnaK_p5	CTTTCAGGGGGAATTGCTGT	CAGCAGGATCATTCACACGA	50.0	50.0	58.1	58.0	2970 bp	1.0	80.9