Hotspot

psaC-ycf35

IRa · 112497-112588 · 92 bp

Back to Stephanodiscus

Pi 0.058

MarkerSeek score 71.30

Primer available yes

Label psaC-ycf35

Feature Metadata

label_name: psaC-ycf35
feature_type: igs
region: IRa
length_bp: 92
variable_sites: 8
pi: 0.058
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ycf35_p1	CTTCATCACCACGCGTAGAA	TGGAAGTAATGCACGAACGT	50.0	45.0	58.0	57.8	938 bp	1.0	80.2
psaC-ycf35_p2	TCACCACGCGTAGAAGATTG	TGGAAGTAATGCACGAACGT	50.0	45.0	58.0	57.8	933 bp	1.0	80.2
psaC-ycf35_p3	TTCATCACCACGCGTAGAAG	TGGAAGTAATGCACGAACGT	50.0	45.0	58.0	57.8	937 bp	1.0	80.2
psaC-ycf35_p4	ATTGTGTTGGGTGTAAGCGT	TGGAAGTAATGCACGAACGT	45.0	45.0	58.0	57.8	917 bp	1.0	80.1
psaC-ycf35_p5	TGGGTGTAAGCGTTGTGAAA	TGGAAGTAATGCACGAACGT	45.0	45.0	58.0	57.8	910 bp	1.0	80.0