Hotspot

ftsH-trnT(tgt)

LSC · 49220-49485 · 266 bp

Back to Stephanodiscus

Pi 0.0276

MarkerSeek score 57.00

Primer available yes

Label ftsH-trnT(tgt)

Feature Metadata

label_name: ftsH-trnT(tgt)
feature_type: igs
region: LSC
length_bp: 266
variable_sites: 11
pi: 0.0276
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ftsH-trnT(tgt)_p1	GCTCGTAAAATTTGTTGTCCCA	AACCCCCAACCTACGGATTA	40.9	50.0	57.8	58.0	343 bp	1.0	64.5
ftsH-trnT(tgt)_p2	TGCTCGTAAAATTTGTTGTCCC	AACCCCCAACCTACGGATTA	40.9	50.0	57.8	58.0	344 bp	1.0	64.5
ftsH-trnT(tgt)_p3	GCTCGTAAAATTTGTTGTCCCA	TCTACCGTTGAGCTACACCA	40.9	50.0	57.8	58.1	311 bp	1.0	64.3
ftsH-trnT(tgt)_p4	TGCTCGTAAAATTTGTTGTCCC	TCTACCGTTGAGCTACACCA	40.9	50.0	57.8	58.1	312 bp	1.0	64.3
ftsH-trnT(tgt)_p5	GCTCGTAAAATTTGTTGTCCCA	ATTTGAACCCCCAACCTACG	40.9	50.0	57.8	57.8	348 bp	1.0	63.0