Hotspot

petA-chlI

LSC · 44930-45443 · 514 bp

Back to Stephanodiscus

Pi 0.0246

MarkerSeek score 53.60

Primer available yes

Label petA-chlI

Feature Metadata

label_name: petA-chlI
feature_type: igs
region: LSC
length_bp: 514
variable_sites: 19
pi: 0.0246
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-chlI_p1	AGCTCAGCACGAATTATAGGA	CGTCACCGTTTACGAAAAGA	42.9	45.0	56.6	56.4	726-727 bp	1.0	50.6
petA-chlI_p2	TGAAAAAGTTCAAGCTGCTGA	CGTCACCGTTTACGAAAAGA	38.1	45.0	56.6	56.4	640-641 bp	1.0	50.6
petA-chlI_p3	TGAAAAAGTTCAAGCTGCTGAA	CGTCACCGTTTACGAAAAGA	36.4	45.0	57.3	56.4	640-641 bp	1.0	48.3
petA-chlI_p4	AGCTCAGCACGAATTATAGGA	TCACCGTTTACGAAAAGATCCA	42.9	40.9	56.6	58.0	724-725 bp	1.0	47.1
petA-chlI_p5	AGCTCAGCACGAATTATAGGA	CGTCACCGTTTACGAAAAGATC	42.9	45.5	56.6	58.0	726-727 bp	1.0	46.9