Hotspot

trnV(tac)-trnR(tct)

LSC · 19430-19442 · 13 bp

Back to Stephanodiscus

Pi 0.0513

MarkerSeek score 64.70

Primer available yes

Label trnV(tac)-trnR(tct)

Feature Metadata

label_name: trnV(tac)-trnR(tct)
feature_type: igs
region: LSC
length_bp: 13
variable_sites: 1
pi: 0.0513
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV(tac)-trnR(tct)_p1	AACTCAGCGGTAGAGTGTCT	TGTCCTGATCCGTTAGACGA	50.0	50.0	58.1	57.9	104 bp	1.0	79.1
trnV(tac)-trnR(tct)_p2	GAGTGTCTGCCTTACAAGCA	TGTCCTGATCCGTTAGACGA	50.0	50.0	57.8	57.9	92 bp	1.0	78.6
trnV(tac)-trnR(tct)_p3	GCCTTACAAGCAGAAGGTCA	TGTCCTGATCCGTTAGACGA	50.0	50.0	57.8	57.9	84 bp	1.0	78.5
trnV(tac)-trnR(tct)_p4	TGCCTTACAAGCAGAAGGTC	TGTCCTGATCCGTTAGACGA	50.0	50.0	57.8	57.9	85 bp	1.0	78.5
trnV(tac)-trnR(tct)_p5	CAAGCAGAAGGTCACAGGTT	TAGGCCCAGTAGGAATCGAA	50.0	50.0	58.0	57.5	124 bp	1.0	76.7