Hotspot

ndhF

SSC · 113121-115346 · 2226 bp

Back to Stephania

Pi 0.0187

MarkerSeek score 56.30

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2226
variable_sites: 128
pi: 0.0187
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GAGGTATCAGCAACGACTGG	AGAGAGAGAAAGGCTGGTGA	55.0	50.0	58.1	57.7	2542-2604 bp	0.571429	62.3
ndhF_p2	GAGGTATCAGCAACGACTGG	TGAGAGAGAGAAAGGCTGGT	55.0	50.0	58.1	57.7	2544-2606 bp	0.571429	62.3
ndhF_p3	GGAGGTATCAGCAACGACTG	AGAGAGAGAAAGGCTGGTGA	55.0	50.0	58.1	57.7	2543-2605 bp	0.571429	62.3
ndhF_p4	GAGGTATCAGCAACGACTGG	GGAACCCACTCAATCCCTTC	55.0	55.0	58.1	57.9	2976-3000 bp	0.380952	56.2
ndhF_p5	GGTATCAGCAACGACTGGTT	GGAACCCACTCAATCCCTTC	50.0	55.0	57.9	57.9	2974-2998 bp	0.380952	56.0