Hotspot

ndhG-ndhI

SSC · 122332-122898 · 567 bp

Back to Stephania

Pi 0.0338

MarkerSeek score 70.30

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 567
variable_sites: 63
pi: 0.0338
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	AGAGATAAAAAGTCGAAATGTTTCA	TCCCTTGATTCAAAAACAATTACCA	28.0	32.0	55.5	57.6	562-630 bp	0.714286	37.8
ndhG-ndhI_p2	AGAGATAAAAAGTCGAAATGTTTCA	CCCTTGATTCAAAAACAATTACCA	28.0	33.3	55.5	56.3	561-629 bp	0.714286	37.8
ndhG-ndhI_p3	GAGATAAAAAGTCGAAATGTTTCA	TCCCTTGATTCAAAAACAATTACCA	29.2	32.0	54.2	57.6	561-629 bp	0.714286	37.8
ndhG-ndhI_p4	AGAGATAAAAAGTCGAAATGTTTCAT	TCCCTTGATTCAAAAACAATTACCA	26.9	32.0	56.0	57.6	562-630 bp	0.714286	37.8
ndhG-ndhI_p5	AGAGATAAAAAGTCGAAATGTTTCA	TCCCTTGATTCAAAAACAATTACC	28.0	33.3	55.5	56.1	562-630 bp	0.714286	37.8