Hotspot

trnE-UUC-trnT-GGU

LSC · 33288-34124 · 837 bp

Back to Stephania

Pi 0.0275
MarkerSeek score 77.00
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
837
variable_sites
75
pi
0.0275
unique_haplotype_count
20
primer_available
yes
alignment_reliability
1.00
markerseek_score
77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 TTTGCTAGTACCCTACCCCC GAACCGATGACTTACGCCTT 55.0 50.0 58.1 58.0 943-1117 bp 1.0 88.9
trnE-UUC-trnT-GGU_p2 CGTTGCCTCCTTGAAAGAGA GAACCGATGACTTACGCCTT 50.0 50.0 57.8 58.0 906-1080 bp 1.0 88.7
trnE-UUC-trnT-GGU_p3 TTTGCTAGTACCCTACCCCC TACCGCTGAGTTAAAAGGGC 55.0 50.0 58.1 57.9 908-1082 bp 1.0 88.3
trnE-UUC-trnT-GGU_p4 CGTTGCCTCCTTGAAAGAGA TACCGCTGAGTTAAAAGGGC 50.0 50.0 57.8 57.9 871-1045 bp 1.0 88.1
trnE-UUC-trnT-GGU_p5 TTTGCTAGTACCCTACCCCC CCGATGACTTACGCCTTACC 55.0 55.0 58.1 58.2 940-1114 bp 1.0 87.8