Hotspot

trnD-GUC-trnY-GUA

LSC · 32537-33071 · 535 bp

Back to Stephania

Pi 0.0789
MarkerSeek score 80.30
Primer available yes
Label trnD-GUC-trnY-GUA

Feature Metadata

label_name
trnD-GUC-trnY-GUA
feature_type
igs
region
LSC
length_bp
535
variable_sites
104
pi
0.0789
unique_haplotype_count
14
primer_available
yes
alignment_reliability
1.00
markerseek_score
80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-trnY-GUA_p1 GTGCTCTGACCGATTGAACT TCTACGCTGGTTCAAATCCG 50.0 50.0 57.9 58.0 593 bp 1.0 90.8
trnD-GUC-trnY-GUA_p2 AATCCTAGGTCGGGACTGAC TCTACGCTGGTTCAAATCCG 55.0 50.0 58.2 58.0 649 bp 1.0 89.4
trnD-GUC-trnY-GUA_p3 CAATCCTAGGTCGGGACTGA TCTACGCTGGTTCAAATCCG 55.0 50.0 58.2 58.0 650 bp 1.0 89.4
trnD-GUC-trnY-GUA_p4 ATCCAATCCTAGGTCGGGAC TCTACGCTGGTTCAAATCCG 55.0 50.0 58.3 58.0 653 bp 1.0 88.9
trnD-GUC-trnY-GUA_p5 CATCCAATCCTAGGTCGGGA TCTACGCTGGTTCAAATCCG 55.0 50.0 58.3 58.0 654 bp 1.0 88.9