Hotspot

trnS-GCU-trnG-UCC

LSC · 8830-9976 · 1147 bp

Back to Stephania

Pi 0.0289

MarkerSeek score 69.90

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 1147
variable_sites: 107
pi: 0.0289
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ACGGAAAGAGAGGGATTCGA	TACCACTAAACTATACCCGC	50.0	45.0	58.2	54.0	1256-1342 bp	1.0	57.5
trnS-GCU-trnG-UCC_p2	ACGGAAAGAGAGGGATTCGA	TTACCACTAAACTATACCCGC	50.0	42.9	58.2	54.9	1257-1343 bp	1.0	56.4
trnS-GCU-trnG-UCC_p3	ATTAGCAATCCGCCGCTTTA	TACCACTAAACTATACCCGC	45.0	45.0	58.0	54.0	1207-1293 bp	0.904762	54.4
trnS-GCU-trnG-UCC_p4	ATTAGCAATCCGCCGCTTTA	TTACCACTAAACTATACCCGC	45.0	42.9	58.0	54.9	1208-1294 bp	0.904762	53.3
trnS-GCU-trnG-UCC_p5	ATTAGCAATCCGCCGCTTTA	TTTACCACTAAACTATACCCGC	45.0	40.9	58.0	55.6	1209-1295 bp	0.904762	51.6