Hotspot

psbA-trnK-UUU

LSC · 1666-2044 · 379 bp

Back to Stephania

Pi 0.0298

MarkerSeek score 68.80

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 379
variable_sites: 42
pi: 0.0298
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	TACAGAAGCGACCCCATAGG	CTAGTTCCGGGTTCGAATCC	55.0	55.0	58.6	57.8	461-508 bp	1.0	84.5
psbA-trnK-UUU_p2	TTTTCGGTGCTGGTTATCCA	CTAGTTCCGGGTTCGAATCC	45.0	55.0	57.4	57.8	483-530 bp	1.0	84.4
psbA-trnK-UUU_p3	ATCCAGTTACAGAAGCGACC	CTAGTTCCGGGTTCGAATCC	50.0	55.0	57.3	57.8	468-515 bp	1.0	83.7
psbA-trnK-UUU_p4	AGTTACAGAAGCGACCCCAT	CTAGTTCCGGGTTCGAATCC	50.0	55.0	58.7	57.8	464-511 bp	1.0	83.4
psbA-trnK-UUU_p5	CGGTTTTCGGTGCTGGTTAT	CTAGTTCCGGGTTCGAATCC	50.0	55.0	58.8	57.8	486-533 bp	1.0	82.2