Hotspot

rpl14-rpl16

LSC · 78974-79082 · 109 bp

Back to Stenotaphrum

Pi 0.0826

MarkerSeek score 72.10

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 109
variable_sites: 9
pi: 0.0826
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	GATTACCAGCGGCTCCTATG	GCAATGACACGATATGCACG	55.0	50.0	57.9	58.0	455-458 bp	1.0	81.3
rpl14-rpl16_p2	GATTACCAGCGGCTCCTATG	CAGGACGAAGAGCAATGACA	55.0	50.0	57.9	57.9	466-469 bp	1.0	80.7
rpl14-rpl16_p3	TCAGCAATAGTGTCCTTGCC	GCAATGACACGATATGCACG	50.0	50.0	57.9	58.0	898-901 bp	1.0	80.6
rpl14-rpl16_p4	CTCAATTCTTCGGCAATCGC	GCAATGACACGATATGCACG	50.0	50.0	57.9	58.0	687 bp	0.5	59.2
rpl14-rpl16_p5	TTTCTAGAGGCATTTGGGGC	GCAATGACACGATATGCACG	50.0	50.0	57.9	58.0	527 bp	0.5	58.8