Hotspot

rpl36-infA

LSC · 77565-77664 · 100 bp

Back to Stenotaphrum

Pi 0.06

MarkerSeek score 72.70

Primer available yes

Label rpl36-infA

Feature Metadata

label_name: rpl36-infA
feature_type: igs
region: LSC
length_bp: 100
variable_sites: 6
pi: 0.06
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-infA_p1	TGACTCTAATTCGTCCACGC	ATTCGAAGCGTACCGAAGAC	50.0	50.0	57.7	58.1	244 bp	1.0	79.0
rpl36-infA_p2	TGACTCTAATTCGTCCACGC	GATTCGAAGCGTACCGAAGA	50.0	50.0	57.7	57.8	245 bp	1.0	78.3
rpl36-infA_p3	CTCTAATTCGTCCACGCCTG	ATTCGAAGCGTACCGAAGAC	55.0	50.0	58.4	58.1	241 bp	1.0	77.7
rpl36-infA_p4	GACTCTAATTCGTCCACGCC	ATTCGAAGCGTACCGAAGAC	55.0	50.0	58.4	58.1	243 bp	1.0	77.7
rpl36-infA_p5	CTCTAATTCGTCCACGCCTG	GATTCGAAGCGTACCGAAGA	55.0	50.0	58.4	57.8	242 bp	1.0	77.0