Hotspot

rbcL-psaI

LSC · 57920-58800 · 881 bp

Back to Stenotaphrum

Pi 0.0183

MarkerSeek score 56.30

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 881
variable_sites: 16
pi: 0.0183
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	AAGCGATGGATACCCTATAG	GCCATTGCAATTGCTGGAAA	45.0	45.0	53.5	58.2	972-975 bp	1.0	44.1
rbcL-psaI_p2	AAGCGATGGATACCCTATAG	AAGGCACGAAAATAGAGGGT	45.0	45.0	53.5	56.8	938-941 bp	1.0	41.8
rbcL-psaI_p3	AAGCGATGGATACCCTATAG	GAAGCCATTGCAATTGCTGG	45.0	50.0	53.5	58.6	975-978 bp	1.0	41.7
rbcL-psaI_p4	AAGCGATGGATACCCTATAG	AAGCCATTGCAATTGCTGGA	45.0	45.0	53.5	59.0	974-977 bp	1.0	41.7
rbcL-psaI_p5	AAGCGATGGATACCCTATAG	AGCCATTGCAATTGCTGGAA	45.0	45.0	53.5	59.0	973-976 bp	1.0	41.7