Hotspot

atpB-rbcL

LSC · 55762-56491 · 730 bp

Back to Stenotaphrum

Pi 0.0152

MarkerSeek score 67.10

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 730
variable_sites: 11
pi: 0.0152
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CCCGGGGGAAAAGTGATATC	ACTCCGGGGTGTAGTAAGTC	55.0	55.0	57.7	58.2	928-1008 bp	1.0	78.1
atpB-rbcL_p2	CCCGGGGGAAAAGTGATATC	CTCCGGGGTGTAGTAAGTCA	55.0	55.0	57.7	58.2	927-1007 bp	1.0	78.1
atpB-rbcL_p3	CCCGGGGGAAAAGTGATATC	CGTACTCCGGGGTGTAGTAA	55.0	55.0	57.7	58.2	931-1011 bp	1.0	77.3
atpB-rbcL_p4	TGATATCTAGCACGGGTCCA	ACTCCGGGGTGTAGTAAGTC	50.0	55.0	57.6	58.2	915-995 bp	1.0	77.3
atpB-rbcL_p5	TGATATCTAGCACGGGTCCA	CTCCGGGGTGTAGTAAGTCA	50.0	55.0	57.6	58.2	914-994 bp	1.0	77.3