Hotspot

psaA-ycf3

LSC · 43507-44144 · 638 bp

Back to Stenotaphrum

Pi 0.0113

MarkerSeek score 64.60

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 638
variable_sites: 7
pi: 0.0113
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GTCTGGCCCATTCCTCAAAA	TATAGCGCTTACTCCGGGAA	50.0	50.0	58.1	58.0	790-799 bp	1.0	81.7
psaA-ycf3_p2	GTCTGGCCCATTCCTCAAAA	GCGCTTACTCCGGGAAATTA	50.0	50.0	58.1	58.1	786-795 bp	1.0	81.4
psaA-ycf3_p3	CTGGCCCATTCCTCAAAAGA	TATAGCGCTTACTCCGGGAA	50.0	50.0	57.8	58.0	788-797 bp	1.0	80.5
psaA-ycf3_p4	TCTGGCCCATTCCTCAAAAG	TATAGCGCTTACTCCGGGAA	50.0	50.0	57.8	58.0	789-798 bp	1.0	80.5
psaA-ycf3_p5	CTGGCCCATTCCTCAAAAGA	GCGCTTACTCCGGGAAATTA	50.0	50.0	57.8	58.1	784-793 bp	1.0	80.2