Hotspot

trnG-UCC-trnM-CAU

LSC · 13986-15926 · 1941 bp

Back to Stenotaphrum

Pi 0.0216

MarkerSeek score 62.90

Primer available yes

Label trnG-UCC-trnM-CAU

Feature Metadata

label_name: trnG-UCC-trnM-CAU
feature_type: igs
region: LSC
length_bp: 1941
variable_sites: 42
pi: 0.0216
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnM-CAU_p1	ACGAATCACACTTTTACCACT	ATTCGAACCGATGACTTCCG	38.1	50.0	55.5	58.1	2095-2106 bp	1.0	52.7
trnG-UCC-trnM-CAU_p2	ACGAATCACACTTTTACCACT	CCGATGACTTCCGTCTTACC	38.1	55.0	55.5	57.8	2088-2099 bp	1.0	51.7
trnG-UCC-trnM-CAU_p3	AGAACGAATCACACTTTTACCAC	ATTCGAACCGATGACTTCCG	39.1	50.0	57.3	58.1	2098-2109 bp	1.0	51.7
trnG-UCC-trnM-CAU_p4	GAACGAATCACACTTTTACCACT	ATTCGAACCGATGACTTCCG	39.1	50.0	57.3	58.1	2097-2108 bp	1.0	51.7
trnG-UCC-trnM-CAU_p5	AACGAATCACACTTTTACCACT	ATTCGAACCGATGACTTCCG	36.4	50.0	56.2	58.1	2096-2107 bp	1.0	50.8