Hotspot

trnG-GCC-trnfM-CAU

LSC · 12702-13063 · 362 bp

Back to Stenotaphrum

Pi 0.0232

MarkerSeek score 63.60

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 362
variable_sites: 8
pi: 0.0232
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	GGCGTAGTTCAATGTAGCGA	CGAGGCTCATAACCTTGAGG	50.0	55.0	58.1	57.8	492-496 bp	1.0	81.5
trnG-GCC-trnfM-CAU_p2	GGCGTAGTTCAATGTAGCGA	CAGTTTGGTAGCTCACGAGG	50.0	55.0	58.1	58.3	507-511 bp	1.0	81.0
trnG-GCC-trnfM-CAU_p3	GGCGTAGTTCAATGTAGCGA	CTGTTTTCGGCCCTACAGAG	50.0	55.0	58.1	58.3	537-541 bp	1.0	80.9
trnG-GCC-trnfM-CAU_p4	GGCGTAGTTCAATGTAGCGA	CTACAGAGCGGAGTAGAGCA	50.0	55.0	58.1	58.3	525-529 bp	1.0	80.5
trnG-GCC-trnfM-CAU_p5	GGCGTAGTTCAATGTAGCGA	TACAGAGCGGAGTAGAGCAG	50.0	55.0	58.1	58.3	524-528 bp	1.0	80.5