Hotspot

psbK-psbI

LSC · 7444-7844 · 401 bp

Back to Stenotaphrum

Pi 0.0274

MarkerSeek score 59.40

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 401
variable_sites: 11
pi: 0.0274
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CAAGCCGCTGTAAGTTTTCG	TTTTTACTCCTCACGCCCAG	50.0	50.0	58.0	57.8	539-544 bp	1.0	79.8
psbK-psbI_p2	AGCCGCTGTAAGTTTTCGAT	TTTTTACTCCTCACGCCCAG	45.0	50.0	57.9	57.8	537-542 bp	1.0	79.4
psbK-psbI_p3	AAGCCGCTGTAAGTTTTCGA	TTTTTACTCCTCACGCCCAG	45.0	50.0	58.1	57.8	538-543 bp	1.0	79.2
psbK-psbI_p4	TTAGCGTTTGTTTGGCAAGC	TTTTTACTCCTCACGCCCAG	45.0	50.0	58.2	57.8	554-559 bp	1.0	78.9
psbK-psbI_p5	GCCGCTGTAAGTTTTCGATG	TTTTTACTCCTCACGCCCAG	50.0	50.0	57.8	57.8	536-541 bp	1.0	78.9