Hotspot

rpl16

LSC · 80397-81953 · 1557 bp

Back to Stenopetalum

Pi 0.0186

MarkerSeek score 61.10

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1557
variable_sites: 51
pi: 0.0186
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	ACACATCAATTCTCGAGCCC	TGCACGTGTTGAATGGATCA	50.0	45.0	58.0	58.1	2005-2107 bp	1.0	85.2
rpl16_p2	GTCTCAATTCCCGTGGGATC	TGCACGTGTTGAATGGATCA	55.0	45.0	58.0	58.1	2264-2366 bp	1.0	84.9
rpl16_p3	ATCGGCGATTACTAGCTCCT	TGCACGTGTTGAATGGATCA	50.0	45.0	58.1	58.1	2036-2138 bp	1.0	84.7
rpl16_p4	ATTGTCTCAATTCCCGTGGG	TGCACGTGTTGAATGGATCA	50.0	45.0	57.9	58.1	2267-2369 bp	1.0	84.2
rpl16_p5	ACACATCAATTCTCGAGCCC	TGGATCAGAGAAGGCAGAGT	50.0	50.0	58.0	57.8	1992-2094 bp	1.0	84.1