Hotspot

orf77-trnL

IRb · 93017-93562 · 546 bp

Back to Stenopetalum

Pi 0.00183

MarkerSeek score 63.60

Primer available yes

Label orf77-trnL

Feature Metadata

label_name: orf77-trnL
feature_type: igs
region: IRb
length_bp: 546
variable_sites: 2
pi: 0.00183
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf77-trnL_p1	CGCATGTCGGATGAAATTGT	AAATGGTAGACACGCGAGAC	45.0	50.0	57.2	58.0	668-677 bp	1.0	76.2
orf77-trnL_p2	ACGCATGTCGGATGAAATTG	AAATGGTAGACACGCGAGAC	45.0	50.0	57.2	58.0	669-678 bp	1.0	76.1
orf77-trnL_p3	ACGCATGTCGGATGAAATTG	TGAAATGGTAGACACGCGAG	45.0	50.0	57.2	58.0	671-680 bp	1.0	76.1
orf77-trnL_p4	CGCATGTCGGATGAAATTGT	TGAAATGGTAGACACGCGAG	45.0	50.0	57.2	58.0	670-679 bp	1.0	76.1
orf77-trnL_p5	CGCATGTCGGATGAAATTGT	GCCTTGGTGGTGAAATGGTA	45.0	50.0	57.2	58.1	680-689 bp	1.0	75.5