Hotspot

petA-psbJ

LSC · 62104-63103 · 1000 bp

Back to Stenopetalum

Pi 0.0153

MarkerSeek score 62.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1000
variable_sites: 27
pi: 0.0153
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	CAGGATTAGGTTCATCCCTGT	50.0	47.6	58.0	56.8	1043-1157 bp	1.0	67.8
petA-psbJ_p2	ATCCATTACGTGTCCAAGGC	TCAGGATTAGGTTCATCCCTGT	50.0	45.5	58.0	58.3	1044-1158 bp	1.0	67.5
petA-psbJ_p3	CATTACGTGTCCAAGGCCTT	CAGGATTAGGTTCATCCCTGT	50.0	47.6	58.2	56.8	1040-1154 bp	1.0	66.7
petA-psbJ_p4	CATTACGTGTCCAAGGCCTT	TCAGGATTAGGTTCATCCCTGT	50.0	45.5	58.2	58.3	1041-1155 bp	1.0	66.4
petA-psbJ_p5	ATCCATTACGTGTCCAAGGC	TCAGGATTAGGTTCATCCCTG	50.0	47.6	58.0	56.5	1044-1158 bp	1.0	65.6