Hotspot

psbG-ndhC

LSC · 49662-49727 · 66 bp

Back to Stenopetalum

Pi 0.0505

MarkerSeek score 77.40

Primer available yes

Label psbG-ndhC

Feature Metadata

label_name: psbG-ndhC
feature_type: igs
region: LSC
length_bp: 66
variable_sites: 5
pi: 0.0505
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbG-ndhC_p1	GGCTGGAAAGTCTTGACCAA	CATGGCGAAAAGGAGCATTG	50.0	50.0	58.0	58.1	196-198 bp	1.0	82.0
psbG-ndhC_p2	GGCTGGAAAGTCTTGACCAA	TGGGCAATGAGTTTCGATGT	50.0	45.0	58.0	57.8	284-286 bp	1.0	80.2
psbG-ndhC_p3	GGCTGGAAAGTCTTGACCAA	TGTACTGGGGGTATCTGCTT	50.0	50.0	58.0	57.7	267-269 bp	1.0	79.8
psbG-ndhC_p4	GGCTGGAAAGTCTTGACCAA	GAGTTTCGATGTACTGGGGG	50.0	55.0	58.0	57.7	276-278 bp	1.0	79.4
psbG-ndhC_p5	GGCTGGAAAGTCTTGACCAA	AGTTTCGATGTACTGGGGGT	50.0	50.0	58.0	58.3	275-277 bp	1.0	79.1