Hotspot

trnT-trnL

LSC · 46001-46854 · 854 bp

Back to Stenopetalum

Pi 0.0158

MarkerSeek score 68.90

Primer available yes

Label trnT-trnL

Feature Metadata

label_name: trnT-trnL
feature_type: igs
region: LSC
length_bp: 854
variable_sites: 22
pi: 0.0158
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-trnL_p1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCAATTCC	55.0	55.0	57.9	58.2	854-916 bp	1.0	83.7
trnT-trnL_p2	TATGGAAAAGCCGGCTATCG	CCGTAGCGTCTACCAATTCC	50.0	55.0	57.8	58.2	905-967 bp	1.0	83.4
trnT-trnL_p3	GCGATGCTCTAACCTCTGAG	AGTCCGTAGCGTCTACCAAT	55.0	50.0	57.9	58.2	857-919 bp	1.0	83.0
trnT-trnL_p4	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCAATTCC	50.0	55.0	57.8	58.2	886-948 bp	1.0	82.8
trnT-trnL_p5	TATGGAAAAGCCGGCTATCG	AGTCCGTAGCGTCTACCAAT	50.0	50.0	57.8	58.2	908-970 bp	1.0	82.7