Hotspot

psaA-ycf3

LSC · 41566-42292 · 727 bp

Back to Stenopetalum

Pi 0.0149

MarkerSeek score 66.90

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 727
variable_sites: 18
pi: 0.0149
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	ATTGGTTGACGATCACGAGG	50.0	50.0	57.9	58.0	693-839 bp	1.0	84.8
psaA-ycf3_p2	AGAAATGACCGGGTTTAGCC	ATTGGTTGACGATCACGAGG	50.0	50.0	57.9	58.0	710-856 bp	1.0	84.7
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	AGCACAGAATTGGTTGACGA	50.0	45.0	57.9	57.7	701-847 bp	1.0	82.8
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	AGCCGCTGAGTATTGGAAAC	50.0	50.0	57.9	58.3	755-901 bp	1.0	82.7
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	CAAGCCGCTGAGTATTGGAA	50.0	50.0	57.9	58.3	757-903 bp	1.0	82.6