Hotspot

ycf1

IRb · 111793-117333 · 5541 bp

Back to Stenomesson

Pi 0.217

MarkerSeek score 63.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 5541
variable_sites: 489
pi: 0.217
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.41
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CCGCCCAGTTTATCGACTTT	AGCCACCTCCTCTTCTTCAT	50.0	50.0	58.3	58.0	843 bp	0.5	57.6
ycf1_p2	CCGCCCAGTTTATCGACTTT	CCACTCGGGGAATTGCAATA	50.0	50.0	58.3	57.9	1305 bp	0.5	57.5
ycf1_p3	CCGCCCAGTTTATCGACTTT	CGTATCAAAGCCACCTCCTC	50.0	55.0	58.3	58.1	851 bp	0.5	57.4
ycf1_p4	CCGCCCAGTTTATCGACTTT	TGATTATACCGCGGCGAAAA	50.0	45.0	58.3	58.1	889 bp	0.5	57.4
ycf1_p5	CCGCCCAGTTTATCGACTTT	TTGATTATACCGCGGCGAAA	50.0	45.0	58.3	58.1	890 bp	0.5	57.4