Hotspot

psaC-ndhE

IRa · 128337-128857 · 521 bp

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Pi 0.0843

MarkerSeek score 75.50

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: IRa
length_bp: 521
variable_sites: 37
pi: 0.0843
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	TACATCCGTCCCAGGGTATC	GGGTCACTTCACTGGTTTGT	55.0	50.0	58.0	57.9	1289-1352 bp	1.0	86.2
psaC-ndhE_p2	TACATCCGTCCCAGGGTATC	AGGCGCAATTACTATGGCTC	55.0	50.0	58.0	58.1	1078-1141 bp	1.0	86.1
psaC-ndhE_p3	TACATCCGTCCCAGGGTATC	GTTGGAGATGGGGTCACTTC	55.0	55.0	58.0	57.9	1299-1362 bp	1.0	85.7
psaC-ndhE_p4	TTTCTAGTAACCGGCCAAGC	GGGTCACTTCACTGGTTTGT	50.0	50.0	57.9	57.9	1886-1950 bp	1.0	84.8
psaC-ndhE_p5	TACATCCGTCCCAGGGTATC	GGAAGCAGCTATTGGGCTAG	55.0	55.0	58.0	58.1	715 bp	0.5	59.1