Hotspot

ndhE-rpl32

SSC · 123268-124080 · 813 bp

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Pi 0.125
MarkerSeek score 80.30
Primer available yes
Label ndhE-rpl32

Feature Metadata

label_name
ndhE-rpl32
feature_type
igs
region
SSC
length_bp
813
variable_sites
76
pi
0.125
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.75
markerseek_score
80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhE-rpl32_p1 TCAACTCGTATCAATCAATCT AGAAAAAGCTTTTATGGCAGC 33.3 38.1 52.4 55.6 951 bp 0.5 20.0
ndhE-rpl32_p2 TCAACTCGTATCAATCAATCT AAGAAAAAGCTTTTATGGCAGC 33.3 36.4 52.4 56.4 952 bp 0.5 20.0
ndhE-rpl32_p3 TCAACTCGTATCAATCAATCT TGGCAGCTAAATATCCCTTT 33.3 40.0 52.4 54.4 937 bp 0.5 20.0
ndhE-rpl32_p4 ATCAACTCGTATCAATCAATCT AGAAAAAGCTTTTATGGCAGC 31.8 38.1 53.1 55.6 952 bp 0.5 20.0
ndhE-rpl32_p5 TCAACTCGTATCAATCAATCT TGGCAGCTAAATATCCCTTTT 33.3 38.1 52.4 55.2 937 bp 0.5 20.0