Hotspot

rpl2

IRa · 148554-150035 · 1482 bp

Back to Stenogyne

Pi 0.0009

MarkerSeek score 59.30

Primer available yes

Label rpl2

Feature Metadata

label_name: rpl2
feature_type: gene
region: IRa
length_bp: 1482
variable_sites: 2
pi: 0.0009
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2_p1	TGAACGTATCACAGCTTACTCC	CGCTTCAACCGGGTTATTCT	45.5	50.0	57.9	58.3	1613 bp	1.0	61.1
rpl2_p2	TGAACGTATCACAGCTTACTCC	ATCATTACGCTTCAACCGGG	45.5	50.0	57.9	58.3	1620 bp	1.0	60.6
rpl2_p3	ACGTATCACAGCTTACTCCT	CGCTTCAACCGGGTTATTCT	45.0	50.0	55.7	58.3	1610 bp	1.0	59.5
rpl2_p4	GAACGTATCACAGCTTACTCCT	CGCTTCAACCGGGTTATTCT	45.5	50.0	57.6	58.3	1612 bp	1.0	59.0
rpl2_p5	ACGTATCACAGCTTACTCCT	ATCATTACGCTTCAACCGGG	45.0	50.0	55.7	58.3	1617 bp	1.0	59.0
rpl2_p1	CGCTTCAACCGGGTTATTCT	TGAACGTATCACAGCTTACTCC	50.0	45.5	58.3	57.9	1613 bp	1.0	61.1
rpl2_p2	ATCATTACGCTTCAACCGGG	TGAACGTATCACAGCTTACTCC	50.0	45.5	58.3	57.9	1620 bp	1.0	60.6
rpl2_p3	CGCTTCAACCGGGTTATTCT	ACGTATCACAGCTTACTCCT	50.0	45.0	58.3	55.7	1610 bp	1.0	59.5
rpl2_p4	CGCTTCAACCGGGTTATTCT	GAACGTATCACAGCTTACTCCT	50.0	45.5	58.3	57.6	1612 bp	1.0	59.0
rpl2_p5	ATCATTACGCTTCAACCGGG	ACGTATCACAGCTTACTCCT	50.0	45.0	58.3	55.7	1617 bp	1.0	59.0