Hotspot

petA

LSC · 60270-61232 · 963 bp

Back to Stenogyne

Pi 0.000692

MarkerSeek score 49.70

Primer available yes

Label petA

Feature Metadata

label_name: petA
feature_type: gene
region: LSC
length_bp: 963
variable_sites: 1
pi: 0.000692
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA_p1	AGCAGAATCTTGGACAGGGA	TGATGTCCATAAATCCGCGA	50.0	45.0	58.3	57.1	1062 bp	1.0	70.2
petA_p2	AGCAGAATCTTGGACAGGGA	ATGTCCATAAATCCGCGAGT	50.0	45.0	58.3	57.1	1060 bp	1.0	70.1
petA_p3	GCAGAATCTTGGACAGGGAA	TGATGTCCATAAATCCGCGA	50.0	45.0	57.5	57.1	1061 bp	1.0	68.9
petA_p4	GCAGAATCTTGGACAGGGAA	ATGTCCATAAATCCGCGAGT	50.0	45.0	57.5	57.1	1059 bp	1.0	68.8
petA_p5	AGCAGAATCTTGGACAGGGA	ACCTGATGTCCATAAATCCGC	50.0	47.6	58.3	58.1	1065 bp	1.0	68.2