Hotspot

rpl32-trnL-UAG

SSC · 110005-110740 · 736 bp

Back to Stenogyne

Pi 0.00181

MarkerSeek score 59.90

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 736
variable_sites: 2
pi: 0.00181
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGGCAGCGTTAAAAGCATTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.9	58.0	866-867 bp	1.0	79.5
rpl32-trnL-UAG_p2	GGGCAGCGTTAAAAGCATTT	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.9	58.0	867-868 bp	1.0	79.5
rpl32-trnL-UAG_p3	AAAAAGAAGGGGTATCGGGC	AGCGTGTCTACCGATTTCAC	50.0	50.0	57.9	58.0	882-883 bp	1.0	79.0
rpl32-trnL-UAG_p4	AAAAAGAAGGGGTATCGGGC	CAGCGTGTCTACCGATTTCA	50.0	50.0	57.9	58.0	883-884 bp	1.0	79.0
rpl32-trnL-UAG_p5	GGGCAGCGTTAAAAGCATTT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	57.9	57.9	876-877 bp	1.0	78.6