Hotspot

trnC-GCA-petN

LSC · 27879-28713 · 835 bp

Back to Stenogyne

Pi 0.0012
MarkerSeek score 60.70
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
835
variable_sites
1
pi
0.0012
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 TTTCCCCAGTTCAAATCCGG ACTTCTTCCCCACACTACGA 50.0 50.0 58.1 58.0 549-948 bp 1.0 83.4
trnC-GCA-petN_p2 TTTCCCCAGTTCAAATCCGG TTCTTCCCCACACTACGAGT 50.0 50.0 58.1 58.0 547-946 bp 1.0 83.4
trnC-GCA-petN_p3 GTATCATTTTGGCGGCATGG ACTTCTTCCCCACACTACGA 50.0 50.0 57.9 58.0 601-1000 bp 1.0 82.9
trnC-GCA-petN_p4 GTATCATTTTGGCGGCATGG TTCTTCCCCACACTACGAGT 50.0 50.0 57.9 58.0 599-998 bp 1.0 82.9
trnC-GCA-petN_p5 TTTCCCCAGTTCAAATCCGG CACTTCTTCCCCACACTACG 50.0 55.0 58.1 57.9 550-949 bp 1.0 82.8