Hotspot

rpoB-trnC-GCA

LSC · 26704-27807 · 1104 bp

Back to Stenogyne

Pi 0.00242

MarkerSeek score 60.90

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1104
variable_sites: 4
pi: 0.00242
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CATTGCTTCATTCGCATCCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.9	58.1	1184-1190 bp	1.0	79.0
rpoB-trnC-GCA_p2	CATTGCTTCATTCGCATCCC	TTTGGTAATCAGGCGACACC	50.0	50.0	57.9	58.2	1203-1209 bp	1.0	78.2
rpoB-trnC-GCA_p3	CATTGCTTCATTCGCATCCC	AAAAGGATTTGCAGTCCCCC	50.0	50.0	57.9	58.4	1166-1172 bp	1.0	76.8
rpoB-trnC-GCA_p4	CATTGCTTCATTCGCATCCC	CGCGTTTTTGGTAATCAGGC	50.0	50.0	57.9	58.4	1209-1215 bp	1.0	76.5
rpoB-trnC-GCA_p5	CATTGCTTCATTCGCATCCC	GCGTTTTTGGTAATCAGGCG	50.0	50.0	57.9	58.4	1208-1214 bp	1.0	76.5