Hotspot

atpH-atpI

LSC · 13394-14368 · 975 bp

Back to Stenogyne

Pi 0.00274

MarkerSeek score 60.50

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 975
variable_sites: 4
pi: 0.00274
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	CCCGTCATGTTCCTTGGATT	45.0	50.0	58.0	57.9	1109-1118 bp	1.0	79.0
atpH-atpI_p2	AATAACGGAAGCGGCAGAAA	CCCGTCATGTTCCTTGGATT	45.0	50.0	58.2	57.9	1110-1119 bp	1.0	77.8
atpH-atpI_p3	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.0	58.5	1063-1072 bp	1.0	75.9
atpH-atpI_p4	AGGTCCAATAGAAGCAAGCC	CCCGTCATGTTCCTTGGATT	50.0	50.0	57.6	57.9	1149-1158 bp	1.0	75.8
atpH-atpI_p5	GTCCAATAGAAGCAAGCCCT	CCCGTCATGTTCCTTGGATT	50.0	50.0	57.6	57.9	1147-1156 bp	1.0	75.8