Hotspot

ycf1

Genome · 125386-130872 · 5487 bp

Back to Stenocoelium

Pi 0.00328

MarkerSeek score 47.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: Genome
length_bp: 5487
variable_sites: 18
pi: 0.00328
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGAGTTTCTTGTCATTGATTGGA	TGAAAGCCTGGAGGAAATTCC	34.8	47.6	56.1	58.2	287 bp	1.0	40.8
ycf1_p2	TGTCGATATTGGGTTTAGGA	TGAAAGCCTGGAGGAAATTCC	40.0	47.6	53.4	58.2	337 bp	1.0	40.7
ycf1_p3	TTGTCGATATTGGGTTTAGGA	TGAAAGCCTGGAGGAAATTCC	38.1	47.6	54.3	58.2	338 bp	1.0	40.7
ycf1_p4	TTTGTCGATATTGGGTTTAGGA	TGAAAGCCTGGAGGAAATTCC	36.4	47.6	55.2	58.2	339 bp	1.0	40.7
ycf1_p5	TGTCGATATTGGGTTTAGGAT	TGAAAGCCTGGAGGAAATTCC	38.1	47.6	54.1	58.2	337 bp	1.0	40.7