Hotspot

rpl2

Genome · 85337-86812 · 1476 bp

Back to Stenocoelium

Pi 0.00339

MarkerSeek score 60.70

Primer available yes

Label rpl2

Feature Metadata

label_name: rpl2
feature_type: gene
region: Genome
length_bp: 1476
variable_sites: 5
pi: 0.00339
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2_p1	AACGTGTCACAGCTTACTCC	CGCTTCAACCGGGTTATTCT	50.0	50.0	57.8	58.3	1614-1617 bp	1.0	76.9
rpl2_p2	AACGTGTCACAGCTTACTCC	ATCATTACGCTTCAACCGGG	50.0	50.0	57.8	58.3	1621-1624 bp	1.0	76.4
rpl2_p3	AACGTGTCACAGCTTACTCC	ACGCTTCAACCGGGTTATTC	50.0	50.0	57.8	58.6	1615-1618 bp	1.0	74.6
rpl2_p4	AACGTGTCACAGCTTACTCC	TACGCTTCAACCGGGTTATT	50.0	45.0	57.8	57.2	1616-1619 bp	1.0	72.9
rpl2_p5	AACGTGTCACAGCTTACTCC	TTACGCTTCAACCGGGTTAT	50.0	45.0	57.8	57.2	1617-1620 bp	1.0	72.9