Hotspot

rpl32-trnL-UAG

Genome · 114755-115734 · 980 bp

Back to Stenocoelium

Pi 0.00326

MarkerSeek score 62.90

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: Genome
length_bp: 980
variable_sites: 3
pi: 0.00326
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AGGGATATTGGGCAGCCTTA	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.2	58.0	1043-1104 bp	1.0	82.8
rpl32-trnL-UAG_p2	AGGGATATTGGGCAGCCTTA	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.2	58.0	1044-1105 bp	1.0	82.8
rpl32-trnL-UAG_p3	AGGGATATTGGGCAGCCTTA	CTCGCACTGCTTCCTAAGAG	50.0	55.0	58.2	58.1	1064-1125 bp	1.0	82.2
rpl32-trnL-UAG_p4	AGGGATATTGGGCAGCCTTA	CTCTCGCACTGCTTCCTAAG	50.0	55.0	58.2	58.1	1066-1127 bp	1.0	82.2
rpl32-trnL-UAG_p5	AGGGATATTGGGCAGCCTTA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.2	57.9	1053-1114 bp	1.0	81.9