Hotspot

trnL-CAA-ndhB

Genome · 94133-94705 · 573 bp

Back to Stenocoelium

Pi 0.00873

MarkerSeek score 63.20

Primer available yes

Label trnL-CAA-ndhB

Feature Metadata

label_name: trnL-CAA-ndhB
feature_type: igs
region: Genome
length_bp: 573
variable_sites: 5
pi: 0.00873
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ndhB_p1	GTCTCGCGTGTCTACCATTT	TGCAATTGCTCAGGATACCC	50.0	50.0	58.0	57.9	633 bp	1.0	80.1
trnL-CAA-ndhB_p2	CTCGCGTGTCTACCATTTCA	TGCAATTGCTCAGGATACCC	50.0	50.0	58.0	57.9	631 bp	1.0	80.1
trnL-CAA-ndhB_p3	TACCATTTCACCACCAAGGC	TGCAATTGCTCAGGATACCC	50.0	50.0	58.1	57.9	621 bp	1.0	79.5
trnL-CAA-ndhB_p4	GCGTGTCTACCATTTCACCA	TGCAATTGCTCAGGATACCC	50.0	50.0	58.2	57.9	628 bp	1.0	78.6
trnL-CAA-ndhB_p5	TCTCGCGTGTCTACCATTTC	TGCAATTGCTCAGGATACCC	50.0	50.0	57.7	57.9	632 bp	1.0	78.0