Hotspot

clpP-psbB

Genome · 73047-73505 · 459 bp

Back to Stenocoelium

Pi 0.00883

MarkerSeek score 61.70

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: Genome
length_bp: 459
variable_sites: 4
pi: 0.00883
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	GCACTATACGTCAACCCAGG	CGATACCAAGGCAAACCCAT	55.0	50.0	58.1	58.2	549-554 bp	1.0	79.0
clpP-psbB_p2	CAGGACTTCGAAAAGGCACT	CGATACCAAGGCAAACCCAT	50.0	50.0	58.1	58.2	514-519 bp	1.0	78.7
clpP-psbB_p3	AGGCACTTTTGGAACACCAA	CGATACCAAGGCAAACCCAT	45.0	50.0	58.1	58.2	501-506 bp	1.0	78.6
clpP-psbB_p4	GTCAACCCAGGATGCATCTT	CGATACCAAGGCAAACCCAT	50.0	50.0	57.9	58.2	540-545 bp	1.0	78.5
clpP-psbB_p5	CGGGGAAATACCGTATGACC	CGATACCAAGGCAAACCCAT	55.0	50.0	57.8	58.2	587-592 bp	1.0	78.2