Hotspot

psaJ-rpl33

Genome · 68166-68636 · 471 bp

Back to Stenocoelium

Pi 0.00661

MarkerSeek score 63.30

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: Genome
length_bp: 471
variable_sites: 3
pi: 0.00661
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	ACATATCTTTCCGTGGCACC	GACATCTTTTCCCTTGGCCA	50.0	50.0	58.0	58.1	589-606 bp	1.0	81.8
psaJ-rpl33_p2	GTTCGGGTCTTTAGCAGGTC	GACATCTTTTCCCTTGGCCA	55.0	50.0	58.3	58.1	551-568 bp	1.0	80.0
psaJ-rpl33_p3	ACATATCTTTCCGTGGCACC	TTTCGAACACAACCGGTACA	50.0	45.0	58.0	57.7	630-647 bp	1.0	79.7
psaJ-rpl33_p4	TTCGGGTCTTTAGCAGGTCT	GACATCTTTTCCCTTGGCCA	50.0	50.0	58.4	58.1	550-567 bp	1.0	79.4
psaJ-rpl33_p5	ATGGTTCGGGTCTTTAGCAG	GACATCTTTTCCCTTGGCCA	50.0	50.0	57.6	58.1	554-571 bp	1.0	79.0