Hotspot

trnE-UUC-trnT-GGU

Genome · 31948-32740 · 793 bp

Back to Stenocoelium

Pi 0.00526

MarkerSeek score 65.80

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: Genome
length_bp: 793
variable_sites: 4
pi: 0.00526
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.8	58.0	870-897 bp	1.0	81.9
trnE-UUC-trnT-GGU_p2	GTGTAGTACCCTACCCCCAG	GAACCGATGACTTACGCCTT	60.0	50.0	58.2	58.0	905-932 bp	1.0	81.5
trnE-UUC-trnT-GGU_p3	CGTTGCCTCCTTGAAAGAGA	TACCGCTGAGTTAAAAGGGC	50.0	50.0	57.8	57.9	835-862 bp	1.0	81.3
trnE-UUC-trnT-GGU_p4	GTGTAGTACCCTACCCCCAG	TACCGCTGAGTTAAAAGGGC	60.0	50.0	58.2	57.9	870-897 bp	1.0	80.8
trnE-UUC-trnT-GGU_p5	CGTTGCCTCCTTGAAAGAGA	CCGATGACTTACGCCTTACC	50.0	55.0	57.8	58.2	867-894 bp	1.0	80.8