Hotspot

petN-psbM

Genome · 29759-30709 · 951 bp

Back to Stenocoelium

Pi 0.00738

MarkerSeek score 61.50

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: Genome
length_bp: 951
variable_sites: 7
pi: 0.00738
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATCC	50.0	50.0	58.2	57.9	1125-1311 bp	1.0	78.6
petN-psbM_p2	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATCC	50.0	50.0	57.6	57.9	1064-1250 bp	1.0	77.0
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	GCTACTGCACTGTTCATCCT	50.0	50.0	58.2	57.6	1124-1310 bp	1.0	76.2
petN-psbM_p4	ATGGGGAAGAAGTGGACTCT	GCTACTGCACTGTTCATCCT	50.0	50.0	57.6	57.6	1063-1249 bp	1.0	74.7
petN-psbM_p5	ATAGTAAGTCTCGCTTGGGC	TGCTACTGCACTGTTCATCC	50.0	50.0	57.1	57.9	1126-1312 bp	1.0	72.7