Hotspot

trnK-UUU-rps16

Genome · 5029-5741 · 713 bp

Back to Stenocoelium

Pi 0.00285
MarkerSeek score 61.30
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
Genome
length_bp
713
variable_sites
2
pi
0.00285
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 TCCCTCGCTTCATCCAAATG AGGAACTGTTCGGGATCTTT 50.0 45.0 58.0 56.5 826-837 bp 1.0 68.6
trnK-UUU-rps16_p2 CCGATGGTATGGACGAATCC AGGAACTGTTCGGGATCTTT 55.0 45.0 57.9 56.5 843-854 bp 1.0 68.3
trnK-UUU-rps16_p3 CGATGGTATGGACGAATCCC AGGAACTGTTCGGGATCTTT 55.0 45.0 57.9 56.5 842-853 bp 1.0 68.3
trnK-UUU-rps16_p4 AAAGCCGAGTACTCTACCGT AGGAACTGTTCGGGATCTTT 50.0 45.0 58.2 56.5 782-793 bp 1.0 67.7
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT AGGAACTGTTCGGGATCTTT 50.0 45.0 58.2 56.5 781-792 bp 1.0 67.7