Hotspot

rpl32-ndhF

SSC · 124103-124952 · 850 bp

Back to Stemona

Pi 0.0107

MarkerSeek score 71.40

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 850
variable_sites: 20
pi: 0.0107
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	AGAAAAAGCTTTTACTCCAGC	AGAAACAGAAGTCCAAACCCT	38.1	42.9	54.7	57.0	1012-1058 bp	1.0	44.5
rpl32-ndhF_p2	AAGAAAAAGCTTTTACTCCAGC	AGAAACAGAAGTCCAAACCCT	36.4	42.9	55.5	57.0	1013-1059 bp	1.0	44.5
rpl32-ndhF_p3	AGAAAAAGCTTTTACTCCAGC	TGTTGCTGTTGGAAGAAACA	38.1	40.0	54.7	56.0	1025-1071 bp	1.0	44.4
rpl32-ndhF_p4	AAGAAAAAGCTTTTACTCCAGC	TGTTGCTGTTGGAAGAAACA	36.4	40.0	55.5	56.0	1026-1072 bp	1.0	44.4
rpl32-ndhF_p5	AGAAAAAGCTTTTACTCCAGC	TTGTTGCTGTTGGAAGAAACA	38.1	38.1	54.7	56.8	1026-1072 bp	1.0	44.4