Hotspot

petD-rpoA

LSC · 76420-76573 · 154 bp

Back to Stemona

Pi 0.0198

MarkerSeek score 59.40

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 154
variable_sites: 6
pi: 0.0198
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	GGTACCGCAGTAGCTCTTTG	GCAATTGATTTACCTAAGAATAAGT	55.0	28.0	58.4	53.9	297-307 bp	1.0	45.3
petD-rpoA_p2	GTACCGCAGTAGCTCTTTGG	GCAATTGATTTACCTAAGAATAAGT	55.0	28.0	58.4	53.9	296-306 bp	1.0	45.3
petD-rpoA_p3	CCGCAGTAGCTCTTTGGTTA	GCAATTGATTTACCTAAGAATAAGT	50.0	28.0	57.6	53.9	293-303 bp	1.0	45.3
petD-rpoA_p4	GTACCGCAGTAGCTCTTTGG	GCAATTGATTTACCTAAGAATAAGTT	55.0	26.9	58.4	54.6	296-306 bp	1.0	45.3
petD-rpoA_p5	ATTGGTACCGCAGTAGCTCT	GCAATTGATTTACCTAAGAATAAGT	50.0	28.0	58.5	53.9	300-310 bp	1.0	45.2