Hotspot

rpl33-rps18

LSC · 66495-66661 · 167 bp

Back to Stemona

Pi 0.0557

MarkerSeek score 78.20

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 167
variable_sites: 18
pi: 0.0557
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	CAAGCATACGATTCATGGGGA	ATAATCGATTCGATCCCCCG	47.6	50.0	58.2	57.0	290-316 bp	1.0	68.2
rpl33-rps18_p2	TGTCCCTATTGTCACAAGCA	ATAATCGATTCGATCCCCCG	45.0	50.0	56.8	57.0	304-330 bp	1.0	67.2
rpl33-rps18_p3	CAAGCATACGATTCATGGGGA	TAATCGATTCGATCCCCCGG	47.6	55.0	58.2	59.1	289-315 bp	1.0	67.0
rpl33-rps18_p4	AAGCATACGATTCATGGGGA	ATAATCGATTCGATCCCCCG	45.0	50.0	56.7	57.0	289-315 bp	1.0	66.8
rpl33-rps18_p5	AGCATACGATTCATGGGGAA	ATAATCGATTCGATCCCCCG	45.0	50.0	56.7	57.0	288-314 bp	1.0	66.8