Hotspot

cemA-psbJ

LSC · 61487-62413 · 927 bp

Back to Stemona

Pi 0.0181

MarkerSeek score 70.80

Primer available yes

Label cemA-psbJ

Feature Metadata

label_name: cemA-psbJ
feature_type: igs
region: LSC
length_bp: 927
variable_sites: 32
pi: 0.0181
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA-psbJ_p1	AAAGTTTCGGATTGCCTGGT	GGTTCATATTCCGGGTTGGG	45.0	55.0	58.0	58.3	1025-1068 bp	1.0	83.2
cemA-psbJ_p2	AAAGTTTCGGATTGCCTGGT	CATATTCCGGGTTGGGTTCA	45.0	50.0	58.0	57.6	1021-1064 bp	1.0	82.3
cemA-psbJ_p3	AAAGTTTCGGATTGCCTGGT	GTTCATATTCCGGGTTGGGT	45.0	50.0	58.0	57.6	1024-1067 bp	1.0	82.3
cemA-psbJ_p4	TTCGGATTGCCTGGTATTCC	GGTTCATATTCCGGGTTGGG	50.0	55.0	57.7	58.3	1020-1063 bp	1.0	80.6
cemA-psbJ_p5	AAAGTTTCGGATTGCCTGGT	CGGTTCATATTCCGGGTTGG	45.0	55.0	58.0	58.7	1026-1069 bp	1.0	80.2