Hotspot

atpB-rbcL

LSC · 53304-53906 · 603 bp

Back to Stemona

Pi 0.0108

MarkerSeek score 59.20

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 603
variable_sites: 12
pi: 0.0108
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CGGTGGAAAAAGGACATCCA	CACCAGCTTTGAATCCAACAC	50.0	47.6	58.1	58.0	754-773 bp	1.0	74.0
atpB-rbcL_p2	CGGTGGAAAAAGGACATCCA	ACCAGCTTTGAATCCAACACT	50.0	42.9	58.1	58.1	753-772 bp	1.0	73.8
atpB-rbcL_p3	GTACCGGGCCGATAATTTGA	CACCAGCTTTGAATCCAACAC	50.0	47.6	57.8	58.0	734-753 bp	1.0	72.9
atpB-rbcL_p4	AGTACCGGGCCGATAATTTG	CACCAGCTTTGAATCCAACAC	50.0	47.6	57.8	58.0	735-754 bp	1.0	72.9
atpB-rbcL_p5	CAGTACCGGGCCGATAATTT	CACCAGCTTTGAATCCAACAC	50.0	47.6	57.8	58.0	736-755 bp	1.0	72.9