Hotspot

ndhC-trnV-UAC

LSC · 49063-50351 · 1289 bp

Back to Stemona

Pi 0.0117

MarkerSeek score 58.50

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1289
variable_sites: 30
pi: 0.0117
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCATATTCGTGAAGCAGAAACA	AGAAGGTCTACGGTTCGAGT	36.4	50.0	56.1	57.8	1166-1402 bp	1.0	53.1
ndhC-trnV-UAC_p2	TCATATTCGTGAAGCAGAAACA	GAAGGTCTACGGTTCGAGTC	36.4	55.0	56.1	57.5	1165-1401 bp	1.0	50.4
ndhC-trnV-UAC_p3	TCATATTCGTGAAGCAGAAACA	GTTCGAGTCCGTATAGCCCT	36.4	55.0	56.1	58.7	1154-1390 bp	1.0	49.3
ndhC-trnV-UAC_p4	CATATTCGTGAAGCAGAAACA	AGAAGGTCTACGGTTCGAGT	38.1	50.0	54.6	57.8	1165-1401 bp	1.0	49.1
ndhC-trnV-UAC_p5	TCATATTCGTGAAGCAGAAACAT	AGAAGGTCTACGGTTCGAGT	34.8	50.0	56.6	57.8	1166-1402 bp	1.0	49.1